3rd Edition of Diabetes and Endocrinology World Conference 2027

Abstract

We evaluated the histological changes in the kidneys of HFD induced Type 2 Diabetes rats treated with low (50 mg/kg) and high doses (500 mg/kg) of TRF and evaluated whether structural changes seen correlated with clinical markers: lipid profile and ACR.

Male Sprague dawley rats (30 days old) were randomly divided into 4 experimental groups: (A) - Non Diabetes; (B) - High fat diet (HFD) induced type 2 diabetes (T2D); (C) - HFD treated with low dose (50 mg/kg) TRF; (D) HFD treated with low dose (500 mg/kg). Urine and Blood samples were collected every 4 weeks over an experimental duration of 28 weeks.

We quantitated Lipid profile (total cholesterol, LDL, HDL, triglycerides) and ACR. At the end of the 28th week all rats were sacrificed and the kidneys and brains were         harvested and preserved for histological procedures. Kidney sections were evaluated for glomerular integrity, tubular damage, fibrosis, and inflammatory infiltration using     H&E staining.

TRF-treated diabetic rats showed reduced glomerular hypertrophy, less tubular injury, and decreased inflammatory cell infiltration compared to untreated diabetic rats.  TRF supplementation improved lipid parameters, reducing LDL and triglycerides while increasing HDL. TRF-treated rats exhibited lower ACR, indicating improved renal     function.

Histological improvements were significantly correlated with better lipid profile and ACR reduction, suggesting a mechanistic link between TRF’s lipid-modulating effects      and renal protection.

TRF supplementation preserves renal structure and function in diabetic nephropathy, with histological improvements aligning with better lipid profile and reduced albuminuria. These findings highlight TRF’s potential as an adjunct therapy for early intervention in diabetic kidney disease.